@article{90876,
  keywords = {Protein Binding, Saccharomyces cerevisiae Proteins, Genome, Fungal, Saccharomyces cerevisiae, Telomere, Genomic Instability, Telomere Homeostasis, DNA Breaks, RecQ Helicases},
  author = {Matthew Bochman and Katrin Paeschke and Angela Chan and Virginia Zakian},
  title = {Hrq1, a homolog of the human RecQ4 helicase, acts catalytically and structurally to promote genome integrity.},
  abstract = { Human RecQ4 (hRecQ4) affects cancer and aging but is difficult to study because it is a fusion between a helicase and an essential replication factor. Budding yeast Hrq1 is homologous to the disease-linked helicase domain of RecQ4 and, like hRecQ4, is a robust 3{\textquoteright}-5{\textquoteright} helicase. Additionally, Hrq1 has the unusual property of forming heptameric rings. Cells lacking Hrq1 exhibited two DNA damage phenotypes: hypersensitivity to DNA interstrand crosslinks (ICLs) and telomere addition to DNA breaks. Both activities are rare; their coexistence in a single protein is unprecedented. Resistance to ICLs requires helicase activity, but suppression of telomere addition does not. Hrq1 also affects telomere length by a noncatalytic mechanism, as well as telomerase-independent telomere maintenance. Because Hrq1 binds telomeres in vivo, it probably affects them directly. Thus, the tumor-suppressing activity of RecQ4 could be due to a role in ICL repair and/or suppression of de novo telomere addition. },
  year = {2014},
  journal = {Cell Rep},
  volume = {6},
  pages = {346-56},
  month = {01/2014},
  issn = {2211-1247},
  doi = {10.1016/j.celrep.2013.12.037},
  language = {eng},
}