@article{90886,
  keywords = {Gene Deletion, DNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Intracellular Signaling Peptides and Proteins, Telomere, Recombination, Genetic, Telomere Homeostasis, Telomerase, Rad51 Recombinase},
  author = {Enea Gino Di Domenico and Stefano Mattarocci and Graziella Cimino-Reale and Paola Parisi and Noemi Cifani and Ettore D{\textquoteright}Ambrosio and Virginia Zakian and Fiorentina Ascenzioni},
  title = {Tel1 and Rad51 are involved in the maintenance of telomeres with capping deficiency.},
  abstract = { Vertebrate-like T2AG3 telomeres in tlc1-h yeast consist of short double-stranded regions and long single-stranded overhang (G-tails) and, although based on Tbf1-capping activity, they are capping deficient. Consistent with this idea, we observe Y{\textquoteright} amplification because of homologous recombination, even in the presence of an active telomerase. In these cells, Y{\textquoteright} amplification occurs by different pathways: in Tel1(+) tlc1h cells, it is Rad51-dependent, whereas in the absence of Tel1, it depends on Rad50. Generation of telomeric G-tail, which is cell cycle regulated, depends on the MRX (Mre11-Rad50-Xrs2) complex in tlc1h cells or is MRX-independent in tlc1h tel1Δ mutants. Unexpectedly, we observe telomere elongation in tlc1h lacking Rad51 that seems to act as a telomerase competitor for binding to telomeric G-tails. Overall, our results show that Tel1 and Rad51 have multiple roles in the maintenance of vertebrate-like telomeres in yeast, supporting the idea that they may participate to evolutionary conserved telomere protection mechanism/s acting at uncapped telomeres. },
  year = {2013},
  journal = {Nucleic Acids Res},
  volume = {41},
  pages = {6490-500},
  month = {07/2013},
  issn = {1362-4962},
  doi = {10.1093/nar/gkt365},
  language = {eng},
}