@article{91106,
  keywords = {Transcription Factors, Macromolecular Substances, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, DNA Replication, Cell Survival, S Phase, Replication Origin, RNA, Transfer, Chromosomal Proteins, Non-Histone, Silencer Elements, Transcriptional, Centromere, DNA Helicases, Telomere-Binding Proteins, DNA, Fungal},
  author = {Andreas Ivessa and Brian Lenzmeier and Jessica Bessler and Lara Goudsouzian and Sandra Schnakenberg and Virginia Zakian},
  title = {The Saccharomyces cerevisiae helicase Rrm3p facilitates replication past nonhistone protein-DNA complexes.},
  abstract = { The Saccharomyces cerevisiae RRM3 gene encodes a 5{\textquoteright} to 3{\textquoteright} DNA helicase. While replication of most of the yeast genome was not dependent upon Rrm3p, in its absence, replication forks paused and often broke at an estimated 1400 discrete sites, including tRNA genes, centromeres, inactive replication origins, and transcriptional silencers. These replication defects were associated with activation of the intra-S phase checkpoint. Activation of the checkpoint was critical for viability of rrm3Delta cells, especially at low temperatures. Each site whose replication was affected by Rrm3p is assembled into a nonnucleosomal protein-DNA complex. At tRNA genes and the silent mating type loci, disruption of these complexes eliminated dependence upon Rrm3p. These data indicate that the Rrm3p DNA helicase helps replication forks traverse protein-DNA complexes, naturally occurring impediments that are encountered in each S phase. },
  year = {2003},
  journal = {Mol Cell},
  volume = {12},
  pages = {1525-36},
  month = {12/2003},
  issn = {1097-2765},
  language = {eng},
}