@article{91126,
  keywords = {Base Sequence, Transcription Factors, Protein Binding, DNA-Binding Proteins, Mitosis, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Genes, Fungal, Fluorescent Antibody Technique, DNA Primers, Chromosome Deletion, Telomere, Telomere-Binding Proteins, Chromosomes, Fungal, rap1 GTP-Binding Proteins},
  author = {Mary Kate Alexander and Virginia Zakian},
  title = {Rap1p telomere association is not required for mitotic stability of a C(3)TA(2) telomere in yeast.},
  abstract = { Telomeric DNA usually consists of a repetitive sequence: C(1-3)A/TG(1-3) in yeast, and C(3)TA(2)/T(2)AG(3) in vertebrates. In yeast, the sequence-specific DNA- binding protein Rap1p is thought to be essential for telomere function. In a tlc1h mutant, the templating region of the telomerase RNA gene is altered so that telomerase adds the vertebrate telomere sequence instead of the yeast sequence to the chromosome end. A tlc1h strain has short but stable telomeres and no growth defect. We show here that Rap1p and the Rap1p-associated Rif2p did not bind to a telomere that contains purely vertebrate repeats, while the TG(1-3) single-stranded DNA binding protein Cdc13p and the normally non-telomeric protein Tbf1p did bind this telomere. A chromosome with one entirely vertebrate-sequence telomere had a wild-type loss rate, and the telomere was maintained at a short but stable length. However, this telomere was unable to silence a telomere-adjacent URA3 gene, and the strain carrying this telomere had a severe defect in meiosis. We conclude that Rap1p localization to a C(3)TA(2) telomere is not required for its essential mitotic functions. },
  year = {2003},
  journal = {EMBO J},
  volume = {22},
  pages = {1688-96},
  month = {04/2003},
  issn = {0261-4189},
  doi = {10.1093/emboj/cdg154},
  language = {eng},
}