@article{91231,
  keywords = {Humans, Gene Deletion, Saccharomyces cerevisiae, DNA Replication, DNA Repair, Genes, Fungal, DNA Damage, Recombination, Genetic, Hydroxyurea, Exodeoxyribonucleases, Transformation, Genetic, DNA, Fungal, Chromosome Fragility, Chromosomes, Fungal, Trinucleotide Repeat Expansion, Exodeoxyribonuclease V, Trinucleotide Repeats, Chromosome Breakage, Electrophoresis, Gel, Pulsed-Field},
  author = {Freudenreich and Kantrow and Zakian},
  title = {Expansion and length-dependent fragility of CTG repeats in yeast.},
  abstract = { Expansion of DNA trinucleotide repeats (TNRs) is the causative mutation in a growing number of human genetic diseases. Large expansions of a CTG tract were obtained and shown by genetic and physical assays to be length-dependent sites of chromosome breakage in Saccharomyces cerevisiae. Deletion of RAD27, which encodes a nuclease involved in Okazaki fragment processing, caused length-dependent destabilization of CTG tracts and a substantial increase in expansion frequency. The genetic assay described here can be used to evaluate other factors that induce TNR expansion or chromosome fragility in humans. },
  year = {1998},
  journal = {Science},
  volume = {279},
  pages = {853-6},
  month = {02/1998},
  issn = {0036-8075},
  language = {eng},
}