@article{91341,
  keywords = {DNA, Single-Stranded, Electrophoresis, Gel, Two-Dimensional, Saccharomyces, Repetitive Sequences, Nucleic Acid, Plasmids, S Phase, Telomere, Base Composition, Blotting, Southern, DNA, Fungal, Chromosomes, Fungal, Electrophoresis, Agar Gel},
  author = {Wellinger and Wolf and Zakian},
  title = {Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase.},
  abstract = { Saccharomyces telomeres consist of approximately 300 bp of C1-3A/TG1-3 DNA. Nondenaturing Southern hybridization, capable of detecting approximately 60 to approximately 300 bases of TG1-3 DNA, revealed that yeast telomeres acquired and lost TG1-3 tails, the predicted intermediate in telomere replication, in a cell cycle-dependent manner. TG1-3 tails were also detected on the ends of a linear plasmid isolated from late S phase cells. In addition, a nonlinear form of this plasmid was detected: this structure migrated in two-dimensional agarose gels like a nicked circle of the same size as the linear plasmid, but had considerably more single-stranded character than a conventional nicked circle. The evidence indicates that these circles were formed by telomere-telomere interactions involving the TG1-3 tails. These data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomere-telomere interactions. },
  year = {1993},
  journal = {Cell},
  volume = {72},
  pages = {51-60},
  month = {01/1993},
  issn = {0092-8674},
  language = {eng},
}