@article{91411, keywords = {Base Sequence, Chromosomes, Saccharomyces cerevisiae, Gene Amplification, Plasmids, Genetic Vectors, DNA, Fungal}, author = {Runge and Zakian}, title = {Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation.}, abstract = { The termini of Saccharomyces cerevisiae chromosomes consist of tracts of C1-3A (one to three cytosine and one adenine residue) sequences of approximately 450 base pairs in length. To gain insights into trans-acting factors at telomeres, high-copy-number linear and circular plasmids containing tracts of C1-3A sequences were introduced into S. cerevisiae. We devised a novel system to distinguish by color colonies that maintained the vector at 1 to 5, 20 to 50, and 100 to 400 copies per cell and used it to change the amount of telomeric DNA sequences per cell. An increase in the number of C1-3A sequences caused an increase in the length of telomeric C1-3A repeats that was proportional to plasmid copy number. Our data suggest that telomere growth is inhibited by a limiting factor(s) that specifically recognizes C1-3A sequences and that this factor can be effectively competed for by long tracts of C1-3A sequences at telomeres or on circular plasmids. Telomeres without this factor are exposed to processes that serve to lengthen chromosome ends. }, year = {1989}, journal = {Mol Cell Biol}, volume = {9}, pages = {1488-97}, month = {04/1989}, issn = {0270-7306}, language = {eng}, }