@article{91511,
  keywords = {RNA, Double-Stranded, Saccharomyces cerevisiae, S Phase, G1 Phase, RNA, Fungal},
  author = {Zakian and Wagner and Fangman},
  title = {Yeast L double-stranded ribonucleic acid is synthesized during the G1 phase but not the S phase of the cell cycle.},
  abstract = { The cytoplasm of Saccharomyces cerevisiae contains two major classes of protein-encapsulated double-stranded ribonucleic acids (dsRNA{\textquoteright}s), L and M. Replication of L and M dsRNA{\textquoteright}s was examined in cells arrested in the G1 phase by either alpha-factor, a yeast mating pheromone, or the restrictive temperature for a cell cycle mutant (cdc7). [3H]uracil was added during the arrest periods to cells prelabeled with [14C]uracil, and replication was monitored by determining the ratio of 3H/14C for purified dsRNA{\textquoteright}s. Like mitochondrial deoxyribonucleic acid, both L and M dsRNA{\textquoteright}s were synthesized in the G1 arrested cells. The replication of L dsRNA was also examined during the S phase, using cells synchronized in two different ways. Cells containing the cdc7 mutation, treated sequentially with alpha-factor and then the restrictive temperature, enter a synchronous S phase when transferred to permissive temperature. When cells entered the S phase, synthesis of L dsRNA ceased, and little or no synthesis was detected throughout the S phase. Synthesis of L dsRNA was also observed in G1 phase cells isolated from asynchronous cultures by velocity centrifugation. Again, synthesis ceased when cells entered the S phase. These results indicate that L dsRNA replication is under cell cycle control. The control differs from that of mitochondrial deoxyribonucleic acid, which replicates in all phases of the cell cycle, and from that of 2-micron DNA, a multiple-copy plasmid whose replication is confined to the S phase. },
  year = {1981},
  journal = {Mol Cell Biol},
  volume = {1},
  pages = {673-9},
  month = {08/1981},
  issn = {0270-7306},
  language = {eng},
}