Local chromatin structure at the ribosomal DNA causes replication fork pausing and genome instability in the absence of the S. cerevisiae DNA helicase Rrm3p.
Publication Year
2004
Type
Journal Article
Abstract
Lack of the yeast Rrm3p DNA helicase causes replication defects at multiple sites within ribosomal DNA (rDNA), including at the replication fork barrier (RFB). These defects were unaltered in rrm3 sir2 cells. When the RFB binding Fob1p was deleted, rrm3-generated defects at the RFB were eliminated, but defects at other rDNA sites were not affected. Thus, specific protein-DNA complexes make replication Rrm3p-dependent. Because rrm3-induced increases in recombination and cell cycle length were only partially suppressed in rrm3 fob1 cells, which still required checkpoint and fork restart activities for viability, non-RFB rrm3-induced defects contribute to rDNA fragility and genome instability.
Keywords
cell cycle,
Mutagenesis,
Binding Sites,
Gene Deletion,
DNA-Binding Proteins,
Electrophoresis, Gel, Two-Dimensional,
Saccharomyces cerevisiae Proteins,
Chromatin,
DNA Replication,
Sirtuins,
Recombination, Genetic,
Histone Deacetylases,
DNA, Ribosomal,
Genomic Instability,
DNA Helicases,
Silent Information Regulator Proteins, Saccharomyces cerevisiae,
Sirtuin 2
Journal
Genes Dev
Volume
18
Issue
5
Pages
498-503
Date Published
03/2004
ISSN Number
0890-9369
Alternate Journal
Genes Dev.
PMID
15037547